Georgia Esoteric and Molecular Lab, LLC

PTEN By FISH

Test Ordering Code: 2161

CPT Codes: 88365 x 2

Turnaround Time:

3 - 5 business days

Specimen:

Two to three slides of 3-4 micron thick sections of formalin fixed, paraffin embedded breast tissue blocks transported at 20-25° C. Please include one hematoxylin and eosin stained slide and the pathology report or the tissue selection for assay should be performed by a pathologist. Specimens fixed in any other fixative except formalin will not be accepted.

Clinical Significance:

Recent evidence suggests that a combination of clinical data (patient age, tumor location, etc.) and molecular markers may be important in determining prognosis in patients with Glioblastoma Multiforme. Specifically, the dtermination of "primary" vs. "secondary" glioblastoma may be important in determining prognosis. GEMLab supplements traditional diagnostic methods with analysis of Epidermal Growth Factor Receptor amplification (EGFR) and chromosome 10q deletions (PTEN) as an aid in properly classifying Glioblastomas.

Indications for Testing:

Patients with Astrocytoma, WHO grades III-IV.

Methodology:

Fluorescent in situ Hybridization (FISH). Fluorescently labeled DNA probes representing the PTEN gene and a centromeric probe for chromosome #10 are used to detect deletions and numerical abnormalities of the chromosome.

Reference Interval:

NOT DELETED : PTEN: CEP 10 =1.0:1.0

DELETED: PTEN: CEP 10 < 1.0:1.0

References:

Schmidt MC, Antweiler S, Urban N, et al. Impact of Genotype Morphology on the Prognosis of Glioblastoma. J Neuropathol Exp Neurol 61:321-328, 2002.